ESRRB Rabbit Polyclonal Antibody

SKU: orb574109

Description

Rabbit polyclonal antibody to ESRRB

Images & Validation

• Tested Applications: WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ESRRB
• Target: ESRRB
• Molecular Weight: 48 kDa
• Purification: Affinity Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

ERR2, ERRb, ESRL2, NR3B2, DFNB35, ERRbeta2, ERR beta-2

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. Isoforms of 48 and 63 kDa are observed in some samples. Recommended dilution for this antibody is 1-3 ug/ml.

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. Multiple isoforms contain this peptide sequence from 63 kDa to 48 kDa.

Lane 1: 25 ug mouse ES cells, Lane 2: 25 ug mouse ES cells overexpressing FLAG EsrrB (mouse), Lane 3: 25 ug chromatin fraction CV1 cells, Lane 4: 25 ug chromatin fraction CV1 cells overexpressing FLAG EsrrB (mouse), Lane 5: 25 ug chromatin fraction CV1 cells overexpressing FLAG EsrrB-Deltacter (mouse), Primary Antibody dilution: 1:500, Secondary Antibody: Anti-rabbit-HRP, Secondary Antibody dilution: 1:3000, Gene Name: ESRRB.

WB Suggested Anti-ESRRB Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: MCF7 cell lysate.

Quick Database Links

Gene Symbol

ESRRB

UniProt

O95718

RefSeq (Protein):NP_004443