EPM2AIP1 Antibody

• Catalog Number: orb1249236
• Category: Antibodies
• Description: EPM2AIP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-TRNQHTLSQPLTDEH
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: EPM2AIP1
• UniProt ID: Q7L775
• NCBI: NP_055620.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: EPM2AIP1, EPM2A (laforin) interacting protein 1, F
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing full-length Human EPM2AIP1 (myc and DYKDDDDK tagged), a band of approx. 70kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.Immunohistochemistry:In paraffin embedded Human Heart shows regular staining of sarcoplasmic reticulum in the cardiac fibers. Recommended concentration, 5-10ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Expiration Date: 12 months from date of receipt.

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human EPM2AI with DYKDDDDK tag probed with orb1249236 (1 ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb1249236 (1 mg/ml) in Lane B.

orb1249236 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.