ENOA Antibody (N-term)

SKU: orb1931371

Description

ENOA Antibody (N-term)

Research Area

Cancer Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: WB - 1:1000, FC - 1:10-50, IF - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Monkey

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 47169 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ENO1L1, MBPB1, MPB1

ENOA Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Fluorescent confocal image of Hela cell stained with ENOA Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENOA primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.

ENOA Antibody (N-term) western blot analysis in U251, mouse C2C12, mouse NIH/3T3 cell line and mouse brain tissue lysates (35 ug/lane). This demonstrates the ENOA antibody detected the ENOA protein (arrow).

Quick Database Links

UniProt

P06733