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ENOA Antibody (N-term)
Catalog Number: orb1931371
| Catalog Number | orb1931371 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 amino acids from the N-terminal region of human ENOA. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | P06733 |
| MW | 47169 Da |
| Tested applications | FC, IF, WB |
| Dilution range | IF: 1:10~50, WB: 1:1000, FC: 1:10~50 |
| Clone Number | RB19994 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, Read more... |
| Note | For research use only |
| NCBI | NP_001188412.1, NP_001419.1 |
ENOA Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent confocal image of Hela cell stained with ENOA Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENOA primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.
ENOA Antibody (N-term) western blot analysis in U251, mouse C2C12, mouse NIH/3T3 cell line and mouse brain tissue lysates (35 ug/lane). This demonstrates the ENOA antibody detected the ENOA protein (arrow).
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