ENOA Antibody (C-term)

• Catalog Number: orb1931370
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-434 amino acids from the C-terminal region of human ENOA.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P06733
• MW: 47169 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Clone Number: RB19995
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Alpha-enolase, 2-phospho-D-glycerate hydro-lyase,
• Research Area: Cancer Biology, Metabolism Research, Signal Transd
• Note: For research use only
• NCBI: NP_001188412.1, NP_001419.1
• Expiration Date: 12 months from date of receipt.

ENOA Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ENOA Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

ENOA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ENOA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ENOA Antibody (C-term) western blot analysis in Jurkat, MCF-7, U251 cell line and mouse brain tissue lysates (35 ug/lane). This demonstrates the ENOA antibody detected the ENOA protein (arrow).