ELP2 Antibody (C-term)

• Catalog Number: orb1931366
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ELP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-765 amino acids from the C-terminal region of human ELP2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q6IA86
• MW: 92500 Da
• Tested applications: FC, IF, WB
• Dilution range: IF: 1:100, WB: 1:1000, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB18010
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Elongator complex protein 2, ELP2, SHINC-2, STAT3-
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_001229804.1, NP_001229806.1, NP_060725.1, NP_001229807.1, NP_001229805.1, NP_001229808.1

Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Fluorescent confocal image of SY5Y cells stained with ELP2 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ELP2 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Western blot analysis of ELP2 antibody (C-term) in NCI-H460 cell line lysates (35 ug/lane). ELP2 (arrow) was detected using the purified Pab.