ELMO1 Antibody

• Catalog Number: orb1247562
• Category: Antibodies
• Description: ELMO1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Zebrafish
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: PKEPSNYDFVYDCN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunoprecipitation: A customer found positive results in IP on Human promyelocytic leukemia cells (HL60). This data is from a previous batch, not on sale.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ELMO1
• UniProt ID: Q92556
• NCBI: NP_001034548.1, NP_055615.8, NP_569709.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ELMO1, engulfment and cell motility 1 (ced-12 homo
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunoprecipitation: A customer found positive results in IP on Human promyelocytic leukemia cells (HL60). This data is from a previous batch, not on sale.
• Expiration Date: 12 months from date of receipt.

orb1247562 (0.3 ug/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247562 (0.3 ug/ml) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.