EIF3M Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1172845
• Category: Antibodies
• Description: EIF3M Recombinant Mouse Monoclonal Antibody
• Target: EIF3M
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG2a, Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Tested applications: ICC, WB
• Dilution range: WB=1:500-1000, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: E8E3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Host-Virus Interaction, Virology
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HepG2, PC-3, PC-12, EL4.IL-2, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 43 kDa, Observed MW: 43 kDa.

Cell line: MCF-7, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172845.