EIF3L Recombinant Mouse Monoclonal Antibody

SKU: orb1173040

Description

EIF3L Recombinant Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:2000-10000, ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG2a, Kappa
• Clone No.: B5C12
• Target: EIF3L
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:10000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: 293, 2: Raw264.7, 3: 3T3, 4: HepG2, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 67 kDa, Observed MW: 55-70 kDa.

Cell line: HEK-293, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:200, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173040.

Quick Database Links

Gene Symbol

EIF3L