E2F-1 (phospho Thr433) rabbit pAb

• Catalog Number: orb767911
• Category: Antibodies
• Description: Rabbit polyclonal antibody to E2F-1 (phospho-Thr433)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433. AA range:388-437
• MW: 70
• Tested applications: ELISA, WB
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-E2F1 antibody, anti-RBBP3 antibody, anti-Tran
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using E2F1 (Phospho-Thr433) Antibody. The lane on the right is blocked with the phospho peptide.