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DYRK1A/B (Phospho-Tyr321/273) Antibody

SKU: orb614262

Description

DYRK1A/B (Phospho-Tyr321/273) Antibody

Images & Validation

Tested ApplicationsWB
Dilution RangeWB 1:1000
ReactivityHuman, Mouse, Rat
Application Notes
DYRK1A/B (Phospho-Tyr321/273) Antibody detects endogenous levels of DYRK1A/B only when phosphorylated at Tyr321/273

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenA synthesized peptide derived from human DYRK1A/B (Phospho-Tyr321/273)
TargetDYRK1A/B
Molecular WeightCalculated MW 80kd
PurityPurified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration1.0mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

DYRK1A, DYRK1, DYRK, HMNB, HP86, MNB, MNB/DYRK protein kinase, MRD7, MNBH, Serine/threonine kinase MNB, Yak1-related kinase

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DYRK1A/B (Phospho-Tyr321/273) Antibody

All lanes : DYRK1A/B (Phospho-Tyr321/273) Antibody at 1/500 dilution: Lane 1 : Hela whole cell lysates. Lane 2 : Hela treated with 100nM Calyculin A for 30min whole cell lysates. Lane 3 : HepG2 whole cell lysates. Lane 4 : HepG2 treated with UV for 15min whole cell lysates. Lane 5 : Mouse brain lysates. Lane 6 : Rat brain lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution. Predicted band size: 70 kDa. Observed band size: 70-80 kDa. Exposure time: 5 seconds

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
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DYRK1A/B (Phospho-Tyr321/273) Antibody (orb614262)

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50 μl
$ 260.00
100 μl
$ 360.00
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