DR6 Antibody [Luke-1]

SKU: orb1273951

Description

DR6 Antibody [Luke-1]

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  DR6 antibody can be used to recognize human DR6 by Flow Cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1 μg/mL of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: Luke-1
• Immunogen: DR6 (monoclonal Luke-1) antibody was raised against recombinant human DR6;Fc (aa. 42-335).
• Purification: DR6 (monoclonal Luke-1) antibody was purified to ≥95% in SDS-PAGE.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 0.02% sodium azide
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

O75509