DR6 Antibody [Luke-1]

• Catalog Number: orb1273951
• Category: Antibodies
• Description: DR6 Antibody [Luke-1]
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: Luke-1
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: DR6 (monoclonal Luke-1) antibody was raised against recombinant human DR6;Fc (aa. 42-335).
• Concentration: batch dependent
• Dilution range: DR6 antibody can be used to recognize human DR6 by Flow Cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1 μg/mL of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• UniProt ID: O75509
• Storage: DR6 antibody can be stored at 4°C, stable for one year.
• Buffer/Preservatives: PBS containing 0.02% sodium azide
• Note: For research use only
• Application notes: DR6 antibody can be used to recognize human DR6 by Flow Cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1 μg/mL of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
• Expiration Date: 12 months from date of receipt.