DPH1 Rabbit Polyclonal Antibody

SKU: orb325709

Description

Rabbit polyclonal antibody to DPH1

Research Area

Cancer

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Yeast, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DPH1
• Target: DPH1
• Molecular Weight: 49 kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti DPH2L antibody, anti DPH2L1 antibody, anti FLJ33211 antibody, anti OVCA1 antibody

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 2 ug/mL of the antibody was used in this experiment. Peptide is present in the canonical 49 kDa protein as well as in a 40 kDa isoform.

Rabbit Anti-DPH1 Antibody, Catalog Number: orb325709, Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver, Observed Staining: Cytoplasm (perinuclear) but not nuclear in hepatocytes, strong signal, low tissue distribution, Primary Antibody Concentration: 1:100, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5 – 2.0 sec, Protocol located in Reviews and Data.

WB Suggested Anti-DPH1 Antibody Titration: 0.2-1 ug/mL, Positive Control: MCF7 cell lysate, DPH1 is supported by BioGPS gene expression data to be expressed in MCF7.

Quick Database Links

Gene Symbol

DPH1

UniProt

Q9BZG8

RefSeq (Protein):NP_001374