Dog IgG Fc Antibody Peroxidase Conjugated

SKU: orb346956

Description

Dog IgG F(c) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:50,000 - 1:125,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Reactivity: Canine
• Application Notes:
  Anti-Dog IgG F(c) Peroxidase Conjugated Antibody has been tested by ELISA. This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Dog IgG F(c) fragment
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG, Dog IgG F(c) and Dog Serum. No reaction was observed against Dog IgG F(ab).
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 1.5 mg/mL
• Disclaimer: For research use only

Alternative Names

rabbit anti-Dog IgG F(c) Antibody Peroxidase conjugation, rabbit anti-Dog IgG Fc fragment HRP conjugated Antibody