Dog IgG F(ab')2

SKU: orb2652768

Description

Dog IgG F(ab')2

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Dog IgG F(ab')2 has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments

Key Properties

• Source: Dog
• Biological Origin: Dog
• Isotype: IgG F(ab')2
• Conjugation: Unconjugated
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG, anti-Dog IgG F(ab’)2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti-Pepsin.

Storage & Handling

• Storage: Store vial at 4° C upon receipt. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dog IgG F(ab')2 fragment, Dog IgG Fab2 fragment

SDS PAGE Results of Dog IgG F(ab)2 Fragment. Lane 1: Molecular Weight Marker. Lane 2: Dog IgG F(ab)2 Reduced [1.0 µg]. 4-20% Gel, Coomassie Stained.