Dog IgG Fab Antibody

SKU: orb346081

Description

Dog IgG Fab Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Dog IgG F(ab) fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Key Properties

• Source: Dog
• Biological Origin: Dog
• Isotype: IgG Fab
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG, anti-Dog IgG F(ab’)2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti- Papain.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dog IgG Fab Fragment

SDS-Page of Dog IgG Fab Fragment. Lane 1: Dog Fab – Reduced. Lane 2: Dog Fab – Non-reduced. Load: 1.0 µg per lane. Predicted/Observed size: 25 kDa – Reduced, 50 kDa – Non-reduced for Fab fragment. Other band(s): None.