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Catalog Number | orb346081 |
---|---|
Category | Proteins |
Description | Dog IgG Fab Antibody |
Tested applications | SDS-PAGE |
Isotype | IgG Fab |
Concentration | 1.0 mg/mL |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG, anti-Dog IgG F(ab’)2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti- Papain. |
Conjugation | Unconjugated |
Source | Dog |
Biological Origin | Dog |
Storage | Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Dog IgG Fab Fragment Read more... |
Note | For research use only |
Application notes | Dog IgG F(ab) fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
Expiration Date | 12 months from date of receipt. |
SDS-Page of Dog IgG Fab Fragment. Lane 1: Dog Fab – Reduced. Lane 2: Dog Fab – Non-reduced. Load: 1.0 µg per lane. Predicted/Observed size: 25 kDa – Reduced, 50 kDa – Non-reduced for Fab fragment. Other band(s): None.
ELISA, IHC, WB | |
Canine | |
Rabbit | |
Polyclonal | |
Biotin |
FC, IF | |
Canine | |
Rabbit | |
Polyclonal | |
RPE |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Dog IgG, anti-Dog IgG F(ab’)2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti-Papain. | |
Dog |