Dnmt3a Antibody

SKU: orb1939035

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Gallus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD23074
• Immunogen: This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from human Dnmt3a. Antigen Region: 457-486 aa.
• Target: DNMT3A
• Molecular Weight: 101858 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A

All lanes: Anti-Dnmt3a Antibody at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: Human skeletal muscle tissue lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: Mouse brain tissue lysate. Lane 6: Rat heart tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Dnmt3a Antibody at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: T47D whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: Rat heart tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Lysates from mice thymus tissue after radiation were subjected to WB using antibody against DNMT3a. CT, control animals; FR, animals subjected to fractionated exposure; AC, acutely exposed animals. All sample loading was normalized to protein content. Representative Western blots from three independent experiments are shown; each lane represents a protein extract of a thymus of one animal. (Mol. Cancer Res. 2005 Oct 01;3 (10):553-561)

Western blot analysis of anti-Dnmt3a Pab in T47-D cell lysate. Dnmt3a (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Quick Database Links

Gene Symbol

DNMT3A

UniProt

Q9Y6K1

RefSeq (Protein):NP_783328.1, NP_715640.2, NP_783329.1, NP_072046.2