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Item 1 of 7
Item 1 of 7
DiMethyl-Histone H3-K79 pAb
Catalog Number: orb1258180
| Catalog Number | orb1258180 |
|---|---|
| Category | Antibodies |
| Description | DiMethyl-Histone H3-K79 pAb |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ChIP, IF, IHC, IP, WB |
| Reactivity | Human, Mouse, Other, Rat |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human DiMethyl-Histone H3-K79 |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 17kDa |
| Target | H3K79me2 |
| UniProt ID | Q16695 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | H3F3A, H3t, H3.4, H3/g, H3FT Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K79 antibody (orb1258180). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody (orb1258180).
Immunohistochemistry of paraffin-embedded human mammary cancer using DiMethyl-Histone H3-K79 antibody (orb1258180) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K79 antibody (orb1258180) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K79 antibody (orb1258180) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 antibody (orb1258180). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 antibody (orb1258180) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
