DIG-11-dUTP

• Catalog Number: orb533128
• Category: Tools
• Description: Digoxigenin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Digoxigenin-labeled DNA/cDNA probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxi-dase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Digoxigenin-11-dUTP/dTTP ratio for PCR and Nick Translation: 35% Digoxigenin-11-dUTP/65% dTTP.
• Concentration: 1.0 mM-1.1 mM
• Form/Appearance: filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 1066.91 g/mol (free acid); Detected MW: 1066.34 g/mol (free acid)
• SMILES: C1[C@@H]2[C@](CC[C@@H]1OCC(=O)NCCCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@@H]3O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)[C@@H](O)C3)c1)([C@@H]1[C@@H](CC2)[C@@]2([C@]([C@@H](C1)O)([C@H](CC2)C1=CC(=O)OC1)C)O)C
• Formula: C₄₃H₆₅N₄O₂₁P₃
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I and in-house data; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT); Reverse Transcription with MMLV Reverse Transcriptase. Incorporation into RNA by-3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT). ; Spectroscopic Propertie: λmax 290 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Expiration Date: 12 months from date of receipt.