DIG-11-dUTP

SKU: orb1733878

Description

Digoxigenin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Digoxigenin-labeled DNA/cDNA probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxi-dase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Digoxigenin-11-dUTP/dTTP ratio for PCR and Nick Translation: 35% Digoxigenin-11-dUTP/65% dTTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I and in-house data; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT); Reverse Transcription with MMLV Reverse Transcriptase. Incorporation into RNA by 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT). ; Spectroscopic Propertie: λmax 290 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 1066.91 g/mol (free acid); Detected MW: 1066.34 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₃H₆₅N₄O₂₁P₃
• SMILES: C1[C@@H]2[C@](CC[C@@H]1OCC(=O)NCCCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@@H]3O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)[C@@H](O)C3)c1)([C@@H]1[C@@H](CC2)[C@@]2([C@]([C@@H](C1)O)([C@H](CC2)C1=CC(=O)OC1)C)O)C

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only