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Catalog Number | orb1536416 |
---|---|
Category | Antibodies |
Description | DDX/AKR1C3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C3 (NP_003730.4). MDSKHQCVKLNDGHFMPVLGFGTYAPPEVPRSKALEVTKLAIEAGFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSTFHRPELVRPALENSLKKAQLDYVDLYLIHSPMSLKPGEELSPTDENGKVIFDIVDLCTTWEAMEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNRSKLLDFCKSKDIVLVAYSALGSQRDKRWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTAEDMKAIDGLDRNLHYFNSDSFASHPNYPYSDEY |
Concentration | 1.34 mg/ml |
Dilution range | IHC (1:50 - 1:200), IHC-P (1:200), WB (1:500 - 1:2000) |
Conjugation | Unconjugated |
Target | DDX / AKR1C3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol. PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol |
Alternative names | AKR1C3, 3-alpha-HSD type 2, 17-beta-HSD 5, 3-alpha Read more... |
Note | For research use only |
Application notes | Further information: The predicted MW is 23kDa/36kDa, while the observed MW by Western blot was 35kDa. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of paraffin-embedded rat heart.
Western blot analysis of extracts of various cell lines, using AKR1C3 antibody at 1:1000 dilution.