DDIT4 Antibody (C-term)

• Catalog Number: orb1930139
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB11618
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 25371 Da
• Target: This DDIT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the C-terminal region of human DDIT4.
• UniProt ID: Q9NX09
• NCBI: NP_061931.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: DNA damage-inducible transcript 4 protein, HIF-1 r
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

DDIT4 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

DDIT4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DDIT4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of DDIT4 (arrow) using rabbit polyclonal DDIT4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DDIT4 gene.