DCK Antibody

• Catalog Number: orb1263479
• Category: Antibodies
• Description: DCK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, IP, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the C-terminal region of human DCK.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IP starting dilution is: 1:500~1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: DCK
• UniProt ID: P27707
• NCBI: P27707
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Deoxycytidine kinase, dCK, DCK
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IP starting dilution is: 1:500~1000
• Expiration Date: 12 months from date of receipt.

DCK Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of DCK Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antibody is used in Western blot to detect DCK in mouse intestine tissue lysate.

Deoxycytidine kinase (DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody using the Pierce classic mammalian IP kit reagent. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibody. Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample.