DbpB antibody

• Catalog Number: orb344679
• Category: Antibodies
• Description: DbpB antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-B protein.
• Concentration: 1.1 mg/mL
• Dilution range: ELISA: >1:5,000, WB: 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi DbpB protein. A BLAST analysis was used to suggest cross-reactivity with DbpB from B. burgdorferi and B. garinii sources based on 100% homology with the immunizing sequence. Cross-reactivity with DbpB from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: O50918
• NCBI: NP_045698.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-DbpB Antibody, Decorin-binding protein
• Note: For research use only
• Application notes: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 17.9 kDa in size corresponding to Borrelia burgdorferi DbpB protein by Western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Molecular weight markers MBP-DbpB fusion protein (arrow; expected MW = 60.3 kDa) using DbpB antibody