DAO Antibody

SKU: orb1247623

Description

DAO Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments in human brain (cerebellum, hippocampus, substantia nigra) lysates gave no specific signal but low background (at antibody concentration up to 1µg/mL).Immunohistochemistry:In paraffin embedded Human Cerebellum shows pixulated cytoplasm staining in neurons directly near the granular layer. Recommended concentration: 2-4µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EEKKLSRMPPSH
• Target: DAO
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DAO, D-amino-acid oxidase, DAAO, DAMOX, MGC35381, OXDA

orb1247623 (2 ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247623 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

Quick Database Links

Gene Symbol

DAO

UniProt

P14920

RefSeq (Protein):NP_001908.3