Daidzein

SKU: orb1309277

Description

Daidzein

Research Area

Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 486-66-8
• MW: 254.24
• Purity: 98.84%
• Formula: C₁₅H₁₀O₄
• SMILES: OC1=CC=C(C=C1)C1=COC2=C(C=CC(O)=C2)C1=O
• Target: Endogenous Metabolite,PPAR
• Solubility: DMSO:45.4 mg/mL (178.57 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.87 mM);H2O:< 1 mg/mL (insoluble)

Bioactivity

• In Vivo:
  Daidzein results in reduced body weight, a fact that may be explained by reduced feed consumption in female rats. Daidzein results in slight, but not significant, decreases in ovarian and uterine weights, and mammary gland size in female rats.
• Cell Research:
  Daidzein is dissolved in DMSO and then diluted with appropriate media. HEK293T cells are plated on 24-well plates at a cell density of approximately 2.5×104 cells/well and are grown to 70-80% confluence. Cells are then transiently transfected with a PPAR-α or PPAR-γ expression plasmid, and a plasmid containing the luciferase gene under the control of three tandem PPAR response elements (PPRE × 3 TK-luciferase) using an X-treme GENE HP DNA Transfection Reagent. Renilla luciferase control vectors are co-transfected to control for transfection efficiency. After transfection, cells are cultured for another 24 h in medium containing DMSO or various concentrations (6.25, 12.5, 25 μM) of Daidzein. Cells are lysed, and luciferase activity is measured and expressed as fold induction, that is normalized to the activity of the renilla luciferase control plasmid.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PPAR, Peroxisome proliferator-activated receptors, Daidzein, inhibit, Isoflavone, EndogenousMetabolite, Endogenous Metabolite, Inhibitor

Compound Identifiers

PubChem CID

5281708