cytokeratin 19 Antibody

SKU: orb1248251

Description

cytokeratin 19 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: EIA, ELISA, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 43kDa band observed in Human cancer lysates of Breast, Pancreas and Colon (calculated MW of 44.1kDa according to NP_002267.2). Recommended concentration: 0.03-0.1µg/mL.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as a capturer and as reporter antibody in combination with EB12360

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HTEQLQMSRSEVTD
• Target: cytokeratin 19
• Molecular Weight: Approx 43kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KRT19, keratin 19, CK19, K19, K1CS, 40-kDa keratin intermediate filament, CK-19, cytokeratin 19, cytokeratin-19, keratin, type I cytoskeletal 19, keratin, type I, 40-kd, keratin-19

orb1248251 (0.03 ug/ml) staining of Human Colon cancer lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

cytokeratin 19

UniProt

P08727

RefSeq (Protein):NP_002267.2