CYP3A4 Antibody

SKU: orb1260402

Description

CYP3A4 Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2).
• Target: CYP3A4
• Molecular Weight: Observed: 57kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CYP3A4, cytochrome P450, family 3, subfamily A, polypeptide 4, HGNC:2637, CP33, CP34, CYP3A, CYP3A3, HLP, NF-25, P450C3, P450PCN1, P450-III, steroid inducible, subfamily IIIA (niphedipine oxidase), polypeptide 3, subfamily IIIA, glucocorticoid-inducible P450, nifedipine oxidase

Western blot analysis of extracts of rat liver, using CYP3A4 antibody (orb1260402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver cancer using CYP3A4 antibody (orb1260402) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

CYP3A4

UniProt

P08684