Cycloastragenol

SKU: orb1301678

Description

Cycloastragenol (Astramembrangenin) is a triterpenoid sapogenin derived from Astragalus membranaceus. Research indicates its potential therapeutic role in depression, supported by in vitro and in vivo studies investigating neuroprotective and antidepressant-like mechanisms.

Research Area

Epigenetics & Chromatin, Molecular Biology

Key Properties

• CAS Number: 78574-94-4
• MW: 490.71
• Purity: 99.85% (May vary between batches)
• Formula: C₃₀H₅₀O₅
• SMILES: C[C@]12[C@]3([C@]4([C@]5(C4)[C@@]([C@@H](O)C3)(C(C)(C)[C@@H](O)CC5)[H])CC[C@]1(C)[C@]([C@@H](O)C2)([C@]6(C)O[C@H](C(C)(C)O)CC6)[H])[H]
• Target: Epigenetic Reader Domain,Telomerase
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (6.72 mM);DMSO:125 mg/mL (254.73 mM)

Bioactivity

• In Vivo:
  oral administration of Cyclogalegenol for 7 days attenuated depression-like behavior in experimental mice.Cyclogalegenol stimulates telomerase activity in human neonatal keratinocytes and rat neuronal cells, and induces CREB activation followed by tert and bcl2 expression. Furthermore, Cyclogalegenol may have a novel therapeutic role in depression.
• In Vitro:
  Cyclogalegenol has important anti-inflammatory activity by inhibiting lymphocytes activation, proliferation and cytokines expression, this effect may be related to reduction of overall intracellular Ca2+ overload.
• Cell Research:
  Mouse lymphocytes were obtained from spleens and subjected to Con A for 24 h. Herein, the cells were treated with different concentrations of Cyclogalegenol. Cell viability was assayed by MTT. Pretreated by Cyclogalegenol and stimulated by Con A, the expression of CD69 and CD25, Th1/Th2/Th17 cytokines, cell cycle, proliferation and intracellular Ca2+ concentration ([Ca2+]i) were analyzed by flow cytometry.
• Animal Research:
  Mice were administered Cyclogalegenol 100 mg/kg, or water by oral gavage, or imipramine 15 mg/kg , a currently used tricyclic antidepressant, by intraperitoneal injection for 7 days as well as 45 min before testing. The forced swim test was performed as described previously , by placing a mouse in a glass cylinder (height: 30 cm, diameter: 15 cm) filled with water to a depth of 12 cm. Mice were tested in the cylinder for 6 min. The water was changed before testing another animal. All test sessions were recorded. Depression-like behavior was determined by observing the immobility of the mouse, where immobility is defined as when a mouse floats passively in the water with minimal movement to keep its head above the water. The total period of immobility during the last 4 min of the test session was determined.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cerebral, CREB, Cyclogalegenol, Cycloastragenol, Cyclosieversigenin, ameliorated, bone, Astramembrangenin, Inhibitor, EpigeneticReaderDomain, Epigenetic Reader Domain, neuroinflammation, microglia, loss, inhibit, reperfusion, Telomerase

Compound Identifiers

PubChem CID

13943286