CCNT2 Antibody

SKU: orb750494

Description

CCNT2 antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 74 and 81 kDa in size corresponding to isoforms of Cyclin T2 protein by western blotting in the appropriate tissue or cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-584 of human Cyclin T2a protein.
• Target: CCNT2
• Purity: This antibody is directed against human Cyclin T2 proteins and was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with proteins 74 and 81 kDa in size corresponding to isoforms of Cyclin T2 protein by western blot. A protein:protein BLAST analysis was used to suggest cross reactivity with Cyclin T2a and T2b from dog (96%), bovine (93%), mouse (88%) and rat (85%) sources based on high homology with the full length protein. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Cyclin T2 antibody, CycT2

Western blot using Biorbyt's Anti-Cyclin T2 (Rabbit) is shown to detect two major bands (arrowheads) corresponding to human Cyclin T2a (~74kDa) and T2b (~81kDa) as indicated. Approximately 33 µg of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% BLOTTO (p/n orb348624) in PBS, the membrane was probed for overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).

Quick Database Links

Gene Symbol

CCNT2

UniProt

O60583

RefSeq (Protein):NP_490595