Cyclin A2 Antibody / CCNA2

SKU: orb606541

Description

This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: CCNA2/2333
• Immunogen: Full length protein was used as the immunogen for this CCNA2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human endometrial carcinoma with Cyclin A2 antibody. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

IHC testing of FFPE human endometrial carcinoma with Cyclin A2 antibody. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

SDS-PAGE analysis of purified, BSA-free Cyclin A2 antibody (clone CCNA2/2333) as confirmation of integrity and purity.

Quick Database Links

UniProt

P20248