CXCL10 Antibody

• Catalog Number: orb345077
• Category: Antibodies
• Description: CXCL10 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Goat, Sheep
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 83 amino acids of the mature bovine CXCL10 protein.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:500, WB: 1 µg/mL
• Form/Appearance: Lyophilized
• Purity: This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine CXCL10 protein. A BLAST analysis was used to suggest cross-reactivity with CXCL10 from bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against sheep and goat based on 94-95% homology. Cross-reactivity with CXCL10 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q2KIQ8
• NCBI: NP_001040016
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-CXCL10 Antibody, IP-10, C-X-C motif ch
• Note: For research use only
• Application notes: This protein-A purified CXCL10 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 9.3 kDa in size corresponding to bovine CXCL10 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein-A purified anti-bovine CXCL10 antibody shows detection of recombinant bovine CXCL10 at 9.3 kDa (arrowhead) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (diluted 1:10), and probed with 1 µg/ml primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.