CTX-M rabbit mAb Antibody

SKU: orb1945953

Description

CTX-M (BE33) rabbit mAb

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA 1:1000 Western Blotting 1:1000 Flow not recommended
• Reactivity: E. coli

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: BE33
• Immunogen: recombinant CTX-M antigen
• Purification: Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

ELISA of recombinant CTX-M subtypes (M-14, M-2, M-15) produced by Escherichia coli with anti-CTX-M (C11) rabbit mAb (Cat. orb1945953). ELISA of recombinant CTX-M subtypes (M-14, M-2, M-15) produced by Escherichia coli with anti-CTX-M (C11) rabbit mAb (Cat. orb1945953). This rabbit mAb can recognize multiple CTX-M subtypes.

Detection of CTX-M protein by lateral flow immunoassay using colloidal gold conjugated anti-CTX-M (C11) rabbit mAb (Cat. orb1945953) and goat polyclonal antibody with multiple antigen recognition. The recombinant CTX-M 14 protein produced by E coli was used as standard antigen. Its limit of detection was 0.8 ng/mL.

Western blot analysis of recombinant CTX-M subtypes with anti-CTX-M (C11) rabbit mAb at 0.1 ?g/mL (Cat. orb1945953. HRP-conjugated goat anti rabbit antibody was used as the secondary antibody. Cross-reactivity of the antibody was confirmed by the colorimetric method using TMB. The recombinant CTX-M 2, M14, and M15 protein produced by E. coli was used for analysis.