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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein at 2μg/mL (100μL/well) can bind rhB7-1/ CD80 Protein Fc Chimera with a linear range of 2 - 50 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein at 10μg/mL (100μL/well) can bind rh PD-L1 /B7-H1 /CD274 Protein Fc Chimera with a linear range of 20 - 10000 ng/mL. |
| Target | CTLA4 |
| Tag | His Tag |
| MW | 14.3 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Ala 37 - Phe 162 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
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50 μg, 500 μg, 1 mg, 10 μg

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The purity of rh CTLA4 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_005205.2 |
|---|
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Protocol Information
CTLA 4 Recombinant Protein (orb1227605)
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