CSF1R Rabbit Polyclonal Antibody

SKU: orb6400

Description

CSF1R Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MCSF receptor (201-300/972aa)
• Target: CSF1R
• Molecular Weight: 107 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MCSF Receptor; C FMS; CD 115; CD115; CD115 antigen; CFMS; Colony stimulating factor 1 receptor; Colony stimulating factor I receptor; CSF 1 R; CSF 1R; CSF-1 receptor; CSF-1-R; CSF1 R; CSF1R_HUMAN; CSFR; FIM 2; FIM2; FMS; FMS proto oncogene; FMS protooncogene; HDLS; M-CSF Receptor; M-CSF-R; Macrophage colony stimulating factor 1 receptor; Macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral(v fms) oncogene homolog; Oncogen FMS; Proto-oncogene c-Fms; V-FMS McDonough feline sarcoma viral oncogen homolog, formerly.

Blank control: Hela (blue). Primary Antibody: Rabbit Anti-CD115 antibody (orb6400), dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CD115/MCSF Receptor antibody (orb6400), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MCSF Receptor/CD115 Polyclonal Antibody, Unconjugated (orb6400) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CSF1R

UniProt

P07333