Crotonyl-Histone H2B (Lys11) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1172557
• Category: Antibodies
• Description: Crotonyl-Histone H2B (Lys11) Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: A10A2
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1:2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=0
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + crotonic acid (100 ng/ml, 18hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 14 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 5: Mouse kidney, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 14 kDa.

Tissue: Human breast, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172557.

Tissue: Mouse small intestine, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172557.

Tissue: Rat liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172557.