Collagenase Type III

SKU: orb2815788

Description

Collagenase Type III, a bacterial MMP-13 from Clostridium histolyticum, selectively cleaves type III collagen. Its gentle enzymatic profile makes it suitable for dissociating sensitive tissues, including breast, uterine, and tumor samples, in both in vitro and in vivo research applications.

Research Area

Protein Biochemistry

Key Properties

• Target: MMP
• Solubility: H2O:50 mg/mL

Bioactivity

• In Vitro:
  Collagenase Type III Preparation and Usage Collagenase Type III is dissolved in a buffer (commonly at concentrations of 0.1%-1mg/mL), and incubated with tissue samples at 37°C for 1-2 hours. Cells are then collected by centrifugation and washing. This method is commonly used to prepare primary cells from breast tissue . Experimental Steps: 1. Storage Solution Preparation: (1) Accurately weigh 100mg of collagenase and slowly add it to 1mL of Hank's Balanced Salt Solution (HBSS) containing Ca²⁺ and Mg²⁺. Shake to promote complete dissolution, preparing a 100mg/mL (100×) stock solution. (2) Filter the solution through a 0.22μm filter to sterilize it. Use it immediately or aliquot it into small portions and store at -20°C to -5°C in a light-protected environment to maintain the solution's quality and activity. (3) The required concentration should be determined according to specific experimental conditions or reference literature. Common concentrations for tissue and cell dissociation are 0.5-2.5mg/mL, and for cartilage digestion, concentrations of 1-2mg/mL are typically used. 2. Experimental Method: (1) Clean the target tissue (such as breast tissue, colon cancer stem cells, etc.) thoroughly to remove any impurities. (2) Dissolve Collagenase Type III in an appropriate buffer according to experimental needs, typically at a concentration of 1-2mg/mL. (3) Place the tissue sample in the enzyme solution and incubate at 37°C for 1-2 hours. The incubation time can be adjusted depending on the tissue type and experimental requirements. (4) Add an appropriate amount of stop solution (such as culture medium containing serum) to halt the enzyme activity. (5) Separate the required cells using centrifugation or filtration Methods.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Collagenase Type III

Quick Database Links

Gene Symbol

MMP