COL7A1 Antibody

SKU: orb1246734

Description

COL7A1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Rat
• Predicted Reactivity: Canine, Human, Mouse, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 250kDa band observed in Rat Testis lysates (calculated MW of 159kDa according to Rat NP_001100328.1). Recommended concentration: 0.1-1.5µg/mL.A customer found this antibody unsuccessful on cryosections of Human Skin.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-TVQYSDDPRTEF
• Target: COL7A1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Collagen, type VII, collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive), COL7A1, HGNC:2214, EBD1, EBDCT, EBR1, LC collagen, alpha 1 type VII collagen, collagen VII, alpha-1 polypeptide, long chain collagen, collagen, type VII, alpha 1

orb1246734 (0.5 ug/ml) staining of Rat Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

COL7A1

UniProt

Q02388

RefSeq (Protein):NP_000085.1