COLGALT1 Rabbit Polyclonal Antibody

SKU: orb157169

Description

COLGALT1 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Lipid Metabolism

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Dilution Range: WB=1:500-2000, ELISA=1:5000-10000, ICC/IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GLT25D1 (531-622/622aa)
• Target: COLGALT1
• Molecular Weight: 69 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GLT25D1; FLJ22329; Glycosyltransferase 25 domain containing 1; Hydroxylysine galactosyltransferase 1; Procollagen galactosyltransferase 1; GT251_HUMAN.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-GLT25D1 antibody (orb157169), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Liver tissue lysates, Lane 3: Rat Placenta tissue lysates, Lane 4: Rat Spleen tissue lysates, Lane 5: Rat Liver tissue lysates, Lane 6: Human THP-1 cell lysates, Primary: Anti-COLGALT1 (orb157169) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 69 kDa, Observed band size: 68 kDa.

Quick Database Links

Gene Symbol

COLGALT1

UniProt

Q8NBJ5