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COL4A6 Antibody

SKU: orb1249465

Description

COL4A6 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA
Predicted ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 20kDa band in Human Heart, Kidney and Skeletal Muscle lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 164kDa according to NP_001838.2 and NP_378667.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-PSPEFETETLHNKES
TargetCOL4A6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

COL4A6, collagen, type IV, alpha 6, MGC88184, OTTHUMP00000023834, OTTHUMP00000023835, collagen IV, alpha-6 polypeptide, collagen alpha 6 type IV, collagen of basement membrane, alpha-6, dJ889N15.4 (Collagen Alpha 6(IV)), type IV alpha 6 collagen

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

COL4A6 Antibody (orb1249465)

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0.1 mg
$ 880.00
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