CMA1/Mast Cell Chymase Antibody

• Catalog Number: orb1538251
• Category: Antibodies
• Description: CMA1/Mast Cell Chymase Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Monkey
• Immunogen: Peptide with sequence C-QFRHPKYNTSTLHHD, from the internal region of the protein sequence according to NP_001827.1.
• Concentration: 0.5 mg/ml
• Dilution range: IHC-P (2.5 µg/ml), Peptide-ELISA (1:128000), WB (0.5 - 1 µg/ml)
• Conjugation: Unconjugated
• Target: CMA1 / Mast Cell Chymase
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: CMA1, Chymase, Chymase, heart, Chymase, mast cell,
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B4134 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4134 was determined to be 1.5 to 3.75 ug/ml.
• Expiration Date: 12 months from date of receipt.

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with (0.5 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary