CLIC1 Antibody

SKU: orb614001

Description

CLIC1 antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: WB: 1 µg/mL
• Reactivity: Human
• Application Notes:
  Anti-CLIC1 has been tested in ELISA, Western Blot, and Immunoprecipitation. Expect a band at ~27kda in western blot using appropriate tissues and lysates. Positive controls used in western blot include CLIC1 overexpressing HEK293, WM-115, and HL-60 whole cell lysates.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with human CLIC1 recombinant protein.
• Purity: This product was Protein A purified from monospecific antiserum by chromatography. This antibody is specific for human CLIC1. BLAST analysis was used to suggest cross-reactivity with CLIC1 from human sources based on 100% homology with the immunizing sequence, 98% homology with rat and mouse, and 97% homology with bovine and Oryctolagus cuniculus. Cross-reactivity with CLIC1 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.06 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-CLIC1 Antibody, Chloride Intracellular Channel 1, Regulatory Nuclear Chloride Ion Channel Protein, Chloride Intracellular Channel Protein 1, Nuclear Chloride Ion Channel 27, Chloride Channel ABP, NCC27, HRNCC, G6, Nuclear Chloride Ion Channel Protein, RNCC Protein, P64CLCP

Western Blot of Rabbit Anti-CLIC1 Antibody. Lane 1: Opal Prestained Molecular Weight Markers. Lane 2: CLIC1 Overexpressed HEK293 Lysate (10 µg) [+]. Lane 3: WM115 Lysate (35 µg) [+]. Lane 4: HL-60 Lysate (p/n orb348702) (35 µg) [+]. Lane 5: Human Kidney Lysate (35 µg) [+]. Lane 6: HeLa WCL Lysate (p/n orb348668) (35 µg) [-]. Lane 7: Human Cerebellum Lysate (35 µg) [-]. Primary Antibody: Anti-CLIC1 at 1 µg/ml overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1:70000 for 1hr at RT. Block: BlockOut buffer (p/n orb348644). Expect MW: ~27kDa. Exposure: 22 sec.

Quick Database Links

UniProt

O00299

RefSeq (Protein):NP_001274522.1