CKB Antibody / Creatine Phosphokinase-BB

SKU: orb639589

Description

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Research Area

Epigenetics

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 2-4ug/ml
• Reactivity: Guinea pig, Hamster, Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the CKB antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: CPTC-CKB-2
• Immunogen: Recombinant human full-length protein was used as the immunogen for the CKB antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human HEK293 cell lysate with CKB antibody (clone CPTC-CKB-2). Predicted molecular weight ~43 kDa.

SDS-PAGE analysis of purified, BSA-free CKB antibody (clone CPTC-CKB-2) as confirmation of integrity and purity.

Quick Database Links

UniProt

P12277