Chromogranin A Antibody Cocktail

• Catalog Number: orb749460
• Category: Antibodies
• Description: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CGA/413 + CHGA/777 + CHGA/798
• Tested applications: IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b, kappa + Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the Chromogranin A antibody cocktail.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Chromogranin A antibody cocktail is available for research use only.
• UniProt ID: P10645
• Storage: Store the Chromogranin A antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Chromogranin A antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded parathyroid stained with Chromogranin A antibody (CGA/413 + CHGA/777 + CHGA/798)

IHC: Formalin-fixed, paraffin-embedded pancreas stained with Chromogranin A antibody cocktail

IHC: Formalin-fixed, paraffin-embedded pheochromocytoma stained with Chromogranin A antibody cocktail

IHC: Formalin-fixed, paraffin-embedded adrenal gland stained with Chromogranin A antibody cocktail