Chicken IgM (mu chain) Secondary Antibody

SKU: orb346885

Description

Chicken IgM (mu chain) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Gallus
• Application Notes:
  Anti-Chicken IgM antibody has been tested by ELISA and SDS-PAGE and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Chicken IgM whole molecule
• Purity: Chicken Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against other Chicken heavy or light chain proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Anti-IgM secondary antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

goat anti-Chicken IgM (mu chain) Antibody, goat anti-Chicken IgM mu chain Antibody

SDS-Page of Goat anti-Chicken IgM (Mu Chain) antibody. Lane 1: Gt-a-Chicken IgM Antibody - Non-Reduced. Lane 2: Gt-a-Chicken IgM Antibody - Reduced. Load: 1.0 µg per lane. Predicted/Observed size: 50 kDa, 25 kDa for Reduced. Other band(s): none.

Western Blot of Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n orb346891). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n orb2652774). Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG F(ab) Fragment (p/n orb2652771). Lane 4: Chicken IgM Whole Molecule (p/n orb346054). All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n orb346891) 1:30000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.