Chelidonine

SKU: orb1222219

Description

Chelidonine isolated from Chelidonium majus efficiently induced apoptosis in HeLa cells through possible alteration of p38-p53 and AKT/PI3 kinase signalling pathways. Chelidonine is a promising model compound for overcoming MDR and for enhancing cytotoxicity of chemotherapeutics, especially against leukaemia cells, its efficacy needs to be confirmed in animal models. Chelidonine may be a potential therapeutic agent against metastasis of invasive human cancer cells, exhibits antimigratory and antiinvasive effects in MDA-MB-231 cells, by suppressing COL-induced integrin signaling, through inhibiting the formation of the IPP complex and subsequent downregulation of IPP downstream signaling molecules, such as Akt and ERK1/2.

Key Properties

• CAS Number: 476-32-4
• MW: 353.37
• Purity: >98% (HPLC)
• Formula: C₂₀H₁₉NO₅
• SMILES: CN1CC2=C(C=CC3=C2OCO3)C4C1C5=CC6=C(C=C5CC4O)OCO6
• Target: IDO
• Solubility: In Vitro: DMSO : 100 mg/mL (282.99 mM)

Bioactivity

• In Vitro:
  Chelidonine (5, 10 and 20 μM; 3-4 days) causes lesions and ventral curling in Dugesia japonica; and significantly decreases Djmcm2 expression at 20 μM but no reduction is observed at 5 and 10 μM; as well as prevents cell cycle progression of stem cells. Chelidonine (0-3 μg/mL; 48 h) has cytotoxic activity against melanoma cell lines. Chelidonine (1, 2 and 3 μg/mL; 24 h) decreases mitochondrial membrane potential (MMP) in 50% of A-375 cells at 1 and 1.5 μg/mL, and 62% at 3 μg/mL. Cell Cytotoxicity Assay Cell line: A-375, A-375-p53DD and A-375-p53sh. Concentration: 0-3 μg/mL. Incubation time: 48 h. Result: Exhibited cytotoxic activity against melanoma cell lines with 0.910±0.017 μg/ml, 0.634±0.009 μg/ml and 0.772±0.045 μg/ml in A-375, A-375-p53DD and A-375-p53sh, respectively.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Chelidonine | Stylophorin | Khelidonin | Stylophorin

Quick Database Links

Gene Symbol

IDO