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CDw78 Antibody

SKU: orb750089

Description

This mAb reacts with a carbohydrate determinant, identified as CDw78 (Workshop V; Code CDw78.2). The epitope recognized by this mAb and Leu21 is the same. It is present on some immature and some peripheral blood B cells, and some B cell lines. Its expression is increased after B-cell activation in vitro. CDw78 is also found on tissue macrophages and on epithelial cells, but not on T cells, NK cells, monocytes, granulocytes and myeloid tissues. It appears that CDw78 is a determinant on a major histocompatibility complex (MHC) class II subpopulation.

Images & Validation

Tested ApplicationsFACS, Functional Studies, IF
Dilution RangeFunctional studies (order BSA/sodium azide-free format),Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the CDw78 antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.DF1588
ImmunogenHuman leukocytes were used as the immunogen for the CDw78 antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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CDw78 Antibody

SDS-PAGE Analysis of Purified, BSA-Free CDw78 Antibody (clone DF1588). Confirmation of Integrity and Purity of the Antibody.

UniProt Details

No UniProt data available

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Protocol Information

FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
IF
Immunofluorescence
View Protocol

CDw78 Antibody (orb750089)

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Available Sizes

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20 μg
$ 370.00
100 μg
$ 750.00
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