CDK2 Antibody

SKU: orb750450

Description

CDK2 antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:1,000 - 1:5,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:200 - 1:1,000
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-cdk2 antibody has been tested in western blot and immunohistochemistry and is suitable for IF and other antibody based assays. Multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Use paraffin embedded tissues for immunohistochemistry. Optimal titers for other applications should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Anti-cdk2 antibody was prepared from whole rabbit serum by repeated immunizations with a synthetic peptide corresponding to the C-terminus of the human cdk-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: CDK2
• Purity: Anti-cdk2 antibody was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 33 kDa cdk2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk2 from other species may also occur.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 75 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Cdk2 Antibody, Cdc2 related protein kinase antibody, Cdk 2 antibody, CDKN2 antibody, Cell division kinase 2 antibody, Cell division protein kinase 2 antibody, rabbit anti-Cyclin dependent kinase 2 antibody, p33 protein kinase antibody, cdk

Immunohistochemistry of Anti-CDK2 antibody. Tissue: human skin formalin fixed and paraffin embedded. No pre-treatment of sample was required. Primary Antibody: Anti-CDK2 was diluted 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Rabbit anti-cdk2 was used at a 1:200 dilution to detect cdk-2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 µg of lysate was separated on a 15% SDS-PAGE gel. Cdk-2 is indicated by an arrowhead as a 32-33 kDa band. Note that multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Primary antibody was reacted at room temperature for 1 h. After subsequent washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (orb347654) preceded color development.

Quick Database Links

Gene Symbol

CDK2

UniProt

P24941

RefSeq:CAA43985.1