Recombinant CD81 Antibody

SKU: orb2642685

Description

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Images & Validation

• Tested Applications: ELISA, FACS, IHC-P
• Dilution range: ELISA: order BSA/sodium azide-free format for coating,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant CD81 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: C81/2885R
• Immunogen: A B-cell line derived from a Burkitt lymphoma was used as the immunogen for the recombinant CD81 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone C81/2885R). Gray=unstained cells, Orange = recombinant CD81 antibody.

Flow cytometry staining of human MCF7 cells with recombinant CD81 antibody; Gray=unstained cells, Orange= recombinant CD81 antibody.

IHC staining of FFPE human prostate carcinoma with recombinant CD81 antibody (clone C81/2885R). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling prior to testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD81 antibody (clone C81/2885R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P60033