CD63 Antibody (C-term)

• Catalog Number: orb1930560
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB26813
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 25637 Da
• Target: This CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-190 amino acids from the C-terminal region of human CD63.
• UniProt ID: P08962
• NCBI: NP_001244320.1, NP_001244321.1, NP_001244330.1, NP_001771.1, NP_001244319.1, NP_001244318.1, NP_001244329.1, NP_001254627.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CD63 antigen, Granulophysin, Lysosomal-associated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CD63 Antibody (C-term) at 1:2000 dilution + human plasma lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CD63 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:1000 dilution. Lane 1: A2058 whole cell lysates. Lane 2: MDA-MB-453 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: MDA-MB-453 whole cell lysate. Lane 2: A2058 whole cell lysate. Lane 3: A375 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: MDA-MB-453 whole cell lysate. Lane 2: A2058 whole cell lysate. Lane 3: A375 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: U-251 MG whole cell lysate. Lane 3: THP-1 whole cell lysate. Lane 4: A2058 whole cell lysate. Lane 5: A375 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: A2058 whole cell lysates. Lane 2: A375 whole cell lysates. Lane 3: HL-60 whole cell lysates. Lane 4: THP-1 whole cell lysates. Lane 5: U-87 MG whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.