CD48 Rabbit Polyclonal Antibody

SKU: orb1152083

Description

CD48 Rabbit Polyclonal Antibody

Research Area

Cell Markers, Immunology

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD48 (151-240/240aa)
• Target: CD48
• Molecular Weight: 45 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SLAMF2; Antigen CD48; Antigen CD48; B cell membrane protein; B lymphocyte activation marker BLAST 1 [Precursor]; B lymphocyte activation marker BLAST 1; B-lymphocyte activation marker BLAST-1; BCM 1 surface antigen; BCM1; BCM1 surface antigen; BCM1 surface antigen; BLAST 1; BLAST; BLAST1; BLAST1; CD 48; CD48 antigen(B cell membrane protein); CD48 antigen; CD48 molecule; CD48 protein; CD48_HUMAN; hCD48; Leucocyte antigen MEM 102; Leukocyte antigen MEM-102; mCD48; MEM 102; MEM102; SLAMF 2; TCT.1; TCT.1.

Blank control (blue): mouse spleen cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD48 antibody (orb1152083), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (KLK4 Rabbit pAb) Polyclonal Antibody, Unconjugated (orb1152083) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (KLK4 Rabbit pAb) Polyclonal Antibody, Unconjugated (orb1152083) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (KLK4 Rabbit pAb) Polyclonal Antibody, Unconjugated (orb1152083) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CD48